期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 33, 页码 27462-27474出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b06839
关键词
Immunosensor; reduced graphene oxide; gold nanoparticles; cyclic voltammetry; electrochemical biosensor; interleukin-8
资金
- DST Inspire Faculty project [IFA12-CH-31]
- CSIR-Network project [BSC0112]
- UPoE-II [158,161]
- JNU
- UGC [F.15-1/2016-17/PDFWM-2015-17-UTT-33566(SA-II)]
An efficient electrochemical transducer matrix for biosensing devices requires specific characteristics, such as fast electron transfer, stability, high surface area, biocompatibility, and presence of specific functional groups, to facilitate biomolecule attachment. We demonstrate the fabrication of an electrochemical immunosensor based on a highly stable gold nanoparticles-reduced graphene oxide (AuNPs-rGO) composite material as a transducer matrix for label-free and noninvasive detection of salivary oral cancer biomarker interleukin-8 (IL8). The synergy between rGO and AuNPs allowed the immunosensor to exhibit fast response and high sensitivity due to the improved electron transfer behavior of the composite. The immunosensor shows very fast detection (9 min) of IL8 and high sensitivity with an experimental linear dynamic range of 500 fg mL(-1) to 4 ng mL(-1) and a detection limit of 72.73 +/- 0.18 pg mL(-1). The fabricated immunosensor exhibits excellent specificity toward the detection of IL8 in human saliva samples. Furthermore, the reusability and stability up to 3 months of the immunosensor demonstrates the commercial potential of this nanoplatform for the detection of other biomarkers of clinical relevance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据