期刊
CHEMICAL BIOLOGY & DRUG DESIGN
卷 90, 期 3, 页码 478-484出版社
WILEY
DOI: 10.1111/cbdd.12963
关键词
cucurbitacins; estrone; MAPK pathway; melanoma; Michael addition
资金
- South Dakota Board of Regents BCAAP (Biological Control and Analysis by Applied Photonics) Center
Assembly of cucurbitacin inspired estrone analogs has been previously synthesized and screened against melanoma cell lines. Further synthetic optimization was executed via installation of Azide polar functional moiety across 23, 24 , -unsaturated ketone side chain using Michael addition reaction. This was followed by biological screening against melanoma cell lines employing MTT assay, in-cell-based ELISA assay, and Western blot analysis to monitor the potential of the synthesized analogs to inhibit the phosphorylated ERK levels. This resulted in evolution of MH-4 possessing IC50 of 3.59m with significant decrease in the p-ERK and targeting MAPK pathway.
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