期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 14, 期 3, 页码 2536-2542出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2017.4801
关键词
Roux-en-Y gastric bypass surgery; obesity; hypothalamus; protein tyrosine phosphatase 1B; leptin
资金
- National Clinical Key Specialties Construction of China
- Natural Science Foundation of Chongqing [CSTC2012jjA10040]
- Tackling Project of Science and Technology of Chongqing Committee of Science and Technology [CSTC2012ggyyjs10038]
The present study aimed to explore the effect of Roux-en-Y gastric bypass (RYGB) surgery on protein tyrosine phosphatase 1B (PTP1B) expression levels and leptin activity in hypothalami of obese rats. Obese rats induced by a high-fat diet (HFD) that underwent RYGB (n=11) or sham operation (SO, n=9), as well as an obese control cohort (Obese, n=10) and an additional normal-diet group (ND, n=10) were used. Food efficiency was measured at 8 weeks post-operation. Plasma leptin levels were evaluated and hypothalamic protein tyrosine phosphatase 1B (PTP1B) levels and leptin signaling activity were examined at the genetic and protein levels. The results indicated that food efficiency was typically lower in RYGB rats compared with that in the Obese and SO rats. In the RYGB group, leptin receptor expression and proopiomelanocortin was significantly higher, while Neuropeptide Y levels were lower than those in the Obese and SO groups. Furthermore, the gene and protein expression levels of PTP1B in the RYGB group were lower, while levels of phosphorylafed signal transducer and activator of transcription 3 protein were much higher compared with those in the Obese and SO groups. In conclusion, RYGB surgery significantly suppressed hypothalamic PTP1B protein expression. PTP1B regulation may partially alleviate leptin resistance.
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