期刊
BIOSENSORS & BIOELECTRONICS
卷 98, 期 -, 页码 486-493出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.004
关键词
Bacteria detection; Pathogenic E.coli; Cell-SELEX; DNA aptamer; Bridged Rebar Graphene; Impedimetric aptasensor
类别
资金
- DST Nanomission Project [SR/NM/NS-1510/2014-G]
- INST
- CSIR
- DST, Govt. of India
We report a novel fabrication method of functionalised Bridged Rebar Graphene (BRG) onto newly designed nanostructured aptasensor for label free impedimetric sensing of pathogenic bacteria E. coli O78:K80:H11. The chemical facilitated unscrolling of MWCNT and subsequent bridging with terephthalaldehyde (TPA) to form 3D-hierarchical BRG nanoconstruct exhibited synergistic effect by combining enhanced electrical properties and facile chemical functionality for stable bio-interface. The bacteria-DNA interactions were captured on BRG nanostructured electrode by using specific anti-E.coli DNA aptamer (K-d similar to 14 nM), screened by new in-situ developed SELEX method using phenylboronic acid on microtitre plate. The developed nanostructured aptasensor demonstrated a low detection limit and sensitivity of similar to 10(1) cfu/mL towards E. coli O78:K80:H11 with a dynamic response range from 101 to 106 cfu/mL in water, juice and milk samples.
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