Acidovorax lacteus sp nov., isolated from a culture of a bloom-forming cyanobacterium (Microcystis sp.)

A novel Gram-negative, rod-shaped and motile bacterial strain, designated strain M36(T), was isolated from a culture of a bloom-forming cyanobacterium, Microcystis sp., collected from a eutrophic lake in Korea. Its taxonomic position was investigated by using a polyphasic taxonomic approach. The isolate was found to grow aerobically at 15-42 A degrees C (optimum 25 A degrees C), pH 7.0-11.0 (optimum pH 8.0) and in the presence of 0-1.0% (w/v) NaCl (optimum 0% NaCl) on R2A medium. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain M36(T) is closely related to Acidovorax anthurii DSM 16745(T) (98.1%), Acidovorax konjaci DSM 7481(T) (97.7%) and Acidovorax avenae DSM 7227(T) (97.0%) and also formed a clear phylogenetic lineage with other Acidovorax species. DNA-DNA relatedness between strain M36(T) and the closely related species of the genus Acidovorax was < 30%. The major fatty acid components identified included summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and summed feature 8 (C-18:0 omega 7c and/or C-18:0 omega 6c). The DNA G+C content of strain M36(T) was determined to be 66.8 mol%. Based on above polyphasic evidence, strain M36(T) is concluded to represent a new species of genus Acidovorax, for which the name Acidovorax lacteus sp. nov. is proposed. The type strain is M36(T) (=KCTC 52220(T) = JCM 31890(T)).