期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 110, 期 9, 页码 1189-1197出版社
SPRINGER
DOI: 10.1007/s10482-017-0891-x
关键词
Paenibacillus aquistagni sp nov.; Polyphasic taxonomy; Draft genome; Type IV pilin; KMG-III
类别
资金
- Slovenian Research Agency [P2-0006]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- Federal Public Planning Service-Science Policy, Belgium
Strain 11(T) was isolated from water of an artificial lake accumulating industrial wastewater on the outskirts of Celje, Slovenia. Phenotypic characterisation showed strain 11(T) to be a Gram-stain positive, spore forming bacterium. The 16S rRNA gene sequence identified strain 11(T) as a member of the genus Paenibacillus, closely related to Paenibacillus alvei (96.2%). Genomic similarity with P. alvei 29(T) was 73.1% (gANI), 70.2% (ANIb), 86.7% (ANIm) and 21.7 +/- 2.3% (GGDC). The DNA G+C content of strain 11(T) was determined to be 47.5%. The predominant menaquinone of strain 11(T) was identified as MK-7 and the major fatty acid as anteiso-C-15:0. The peptidoglycan was found to contain meso-diaminopimelic acid. In contrast to its close relatives P. alvei DSM 29(T), Paenibacillus apiarius DSM 5581(T) and Paenibacillus profundus NRIC 0885(T), strain 11(T) was found to be able to ferment d-fructose, d-mannose and d-xylose. A draft genome of strain 11(T) contains a cluster of genes associated with type IV pilin synthesis usually found in clostridia, and only sporadically in other Gram-positive bacteria. Genotypic, chemotaxonomic, physiological and biochemical characteristics of strain 11(T) presented in this study support the creation of a novel species within the genus Paenibacillus, for which the name Paenibacillus aquistagni sp. nov. is proposed, with strain 11(T) (=ZIM B1027(T)aEuroS=LMG 29561(T)aEuroS=CCM 8679(T)aEuroS) as the type strain.
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