Paenibacillus aquistagni sp nov., isolated from an artificial lake accumulating industrial wastewater

Strain 11(T) was isolated from water of an artificial lake accumulating industrial wastewater on the outskirts of Celje, Slovenia. Phenotypic characterisation showed strain 11(T) to be a Gram-stain positive, spore forming bacterium. The 16S rRNA gene sequence identified strain 11(T) as a member of the genus Paenibacillus, closely related to Paenibacillus alvei (96.2%). Genomic similarity with P. alvei 29(T) was 73.1% (gANI), 70.2% (ANIb), 86.7% (ANIm) and 21.7 +/- 2.3% (GGDC). The DNA G+C content of strain 11(T) was determined to be 47.5%. The predominant menaquinone of strain 11(T) was identified as MK-7 and the major fatty acid as anteiso-C-15:0. The peptidoglycan was found to contain meso-diaminopimelic acid. In contrast to its close relatives P. alvei DSM 29(T), Paenibacillus apiarius DSM 5581(T) and Paenibacillus profundus NRIC 0885(T), strain 11(T) was found to be able to ferment d-fructose, d-mannose and d-xylose. A draft genome of strain 11(T) contains a cluster of genes associated with type IV pilin synthesis usually found in clostridia, and only sporadically in other Gram-positive bacteria. Genotypic, chemotaxonomic, physiological and biochemical characteristics of strain 11(T) presented in this study support the creation of a novel species within the genus Paenibacillus, for which the name Paenibacillus aquistagni sp. nov. is proposed, with strain 11(T) (=ZIM B1027(T)aEuroS=LMG 29561(T)aEuroS=CCM 8679(T)aEuroS) as the type strain.