期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 183, 期 1, 页码 293-306出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-017-2445-0
关键词
Glucose isomerase; Fructose; Activity; Immobilization; Stability
资金
- National Natural Science Foundation of China [21602199, 31401527]
- Research Program of Science and Technology Department of Zhejiang Province [2015C32052]
Glucose isomerase is the important enzyme for the production of high fructose corn syrup (HFCS). One-step production of HFCS containing more than 55% fructose (HFCS-55) is receiving much attention for its industrial applications. In this work, the Escherichia coli harboring glucose isomerase mutant TEGI-W139F/V186T was immobilized for efficient production of HFCS-55. The immobilization conditions were optimized, and the maximum enzyme activity recovery of 92% was obtained. The immobilized glucose isomerase showed higher pH, temperature, and operational stabilities with a K (m) value of 272 mM and maximum reaction rate of 23.8 mM min(-1). The fructose concentration still retained above 55% after the immobilized glucose isomerase was reused for 10 cycles, and more than 85% of its initial activity was reserved even after 15 recycles of usage at temperature of 90 A degrees C. The results highlighted the immobilized glucose isomerase as a potential biocatalyst for HFCS-55 production.
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