Heterotrophic Ammonia and Nitrate Bio-removal Over Nitrite (Hanbon): Performance and microflora

A novel (H) under bar eterotrophic (A) under bar mmonia and (N) under bar itrate (B) under bar io-removal (O) under bar ver (N) under bar itrite (Hanbon) process, combining (S) under bar hort (N) under bar itrate (R) under bar eduction (SNR) with (A) under bar naerobic (Amm) under bar onia (Ox) under bar idation (Anammox), was developed in a lab scale continuous up-flow reactor. The substrate effects were investigated to characterize the performance of Hanbon process, and the corresponding microflora information was also revealed. Our results showed that the optimal substrate ratio of NH4+-N:NO3--N:COD for the Hanbon process was 0.65:1:2.2. The volumetric nitrogen removal rate was up to 9.0 +/- 0.1 kgN.m(-3). d(-1) at high influent substrate concentrations of NH4+-N 375 mg L-1, NO3--N 750 mg L-1 and COD 1875 mg L-1, which was superior to the reported values of analogous processes. Moreover, the effluent total nitrogen concentration was able to meet the strict discharge standard (less than 10 mg L-1) at low influent substrate concentration of NH4---N 26 mg L-1, NO3--N 40 mg.L-1 and COD 88 mg L-1. Illumina-based 16S rRNA gene sequencing results showed that Halomonas campisalis and Candidatus Kuenenia stuttgartiensis were the dominant bacteria in the SNR section and Anammox section at high substrate concentration condition. However, Halomonas campaniensis and Candidatus Brocadia brasiliensis were raised significantly at low substrate concentration condition. Hanbon process provided in the present work was flexible of treating wastewater with various nitrogen concentrations, deserving further development. (C) 2017 Elsevier Ltd. All rights reserved.