A comparative study on effects of tadalafil, losartan, extracts of grape seed and ginko biloba on skin inflammation induced by cisplatin in rats

We investigated the effects of cisplatin (CDDP) on inflammation in general and skin inflammation in particular with application of different treatment models using tadalafil (TM) (Tad), losartan (TM) (Los), extracts of grape seed (G.S) and ginko biloba (G.B). Ninety healthy male albino rats were used in this experiment distributed randomly into six groups (n = 15). The duration of the experiment was four weeks. At the end of 1st, 2nd and 4th weeks of treatment, blood samples were subjected to RNA extraction and RTPCR for interleukin (IL)-1B and IL-18 genes expression. The results showed a significant increase (P < 0.0001) in levels of mRNA genes expression of IL-1B and IL-18 in CDDP exposed rats when compared with control rats. Four weeks after treatment, expression fold changes significantly decreased in all treated groups with variable degrees where Los was the best to decrease the fold changes of IL-1B and IL-18 genes expression. Numbers of blood vessels, CD31 positive proliferating endothelial cells around preexisting skin vessels, CD34 positive blood vessels, perivascular inflammatory cells and total skin inflammatory cells were higher in untreated CDDP exposed rats when compared with control rats. Pathological evaluation revealed that Los followed by Tad then G.B were able to reduce the increased inflammatory markers in blood, skin inflammation and skin blood vessel density in CDDP exposed rats.