期刊
METABOLITES
卷 7, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/metabo7030043
关键词
metabolite footprinting; exometabolome; culture medium; metabolic modelling; sample preparation; analytical instruments; data integration; extracellular metabolites; intracellular metabolites
资金
- Agricultural and Marketing Research and Development Trust (AGMARDT) [A16017]
Microorganisms produce and secrete many primary and secondary metabolites to the surrounding environment during their growth. Therefore, extracellular metabolites provide important information about the changes in microbial metabolism due to different environmental cues. The determination of these metabolites is also comparatively easier than the extraction and analysis of intracellular metabolites as there is no need for cell rupture. Many analytical methods are already available and have been used for the analysis of extracellular metabolites from microorganisms over the last two decades. Here, we review the applications and benefits of extracellular metabolite analysis. We also discuss different sample preparation protocols available in the literature for both types (e.g., metabolites in solution and in gas) of extracellular microbial metabolites. Lastly, we evaluate the authenticity of using extracellular metabolomics data in the metabolic modelling of different industrially important microorganisms.
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