Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes

In this study, Sargassum fusiforme polysaccharide was purified using a DEAE-Sepharose fast-flow column to obtain Sargassum fusiforme polysaccharides fraction (SFP-P1). As expected, the SFP-P1 contains mainly carbohydrates (87%) with less than 1% protein. Within the carbohydrate, it contains about 10% sulfate and 18% uronic acids. SFP-P1 consisted of L-fucose (13.17%), galactose (4.28%), glucose (1.95%), xylose (5.50%), and mannose (75.10%) with an average molecular weight of 113 kDa. Mannose and L-fucose were two predominant monosaccharides. In addition, (1)-linked or (1 -> 6) -linked, (1 -> 2) -linked or (1 -> 4) linked and (1 3) -linked glycosyl linkages accounted for 1.62%, 60.69% and 37.69% of all linkages in the molecule, respectively. The glycosidic linkage types of SFP-P1 were proven to be -> 3,6)-alpha-D-Manp (1 4)-alpha-D-GalAp(1 ->, -> 4)-beta-D-Xylp(1 -> and 3,4)-alpha-D-GIcAp(1 The results indicated the SFP-P1 had cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through stimulation of SOD, GSH-P-x enzymes activities and ROS. Also SFP-P1 suppressed the UVB-induced expression of MMP-1 & MMP-9. (C) 2017 Published by Elsevier Ltd.