期刊
JOURNAL OF FUNCTIONAL FOODS
卷 35, 期 -, 页码 352-362出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2017.05.055
关键词
Polysaccharide; Citrus aurantium L. var. amara Engl.; Structure; Immunomodulatory activity; MAPK; NF-kappa B
资金
- Science and Technology Project of Guangzhou City [201604020150]
- Science and Technology Project of Guangdong [2014A020212682]
- Special Research of Traditional Chinese Medicine Hospital of Guangdong Province [YN2014ZH02]
Two polysaccharides CAVAP-I and CAVAP-II from Citrus aurantium L. var. amara Engl showed significant structural differences. Average molecular weights of CAVAP-I and CAVAP-II were 10.5 kDa and 4.5 kDa. Triple-helix conformation existed in CAVAP-II rather than CAVAP-I. Arabinose, mannose, glucose and galactose formed main components of CAVAP-I and CAVAP-II at different ratios. (1 -> 3,6)-beta-D-Gal and (1 -> 5)-alpha-L-Ara composed the backbone of CAVAP-I and CAVAP-II. CAVAP-II displayed greater immune enhancement potential than CAVAP-I. CAVAP-II significantly promoted interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) production, and inducible nitric oxide synthase, IL-6, TNF-alpha and interleukin-1 beta mRNA expression by activating phosphorylated extracellular signal-regulated kinase, c-Jun N-terminal kinase, P38 and P65. These results indicated that structures were closely related to immunomodulatory activity of polysaccharides. CAVAP-II showed the greatest immunostimulation potential followed by CAVAP-I and CAVAPs by activating mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) pathways. (C) 2017 Elsevier Ltd. All rights reserved.
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