期刊
JOURNAL OF FUNCTIONAL FOODS
卷 28, 期 -, 页码 64-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2016.11.005
关键词
Gracilaria rubra; Polysaccharide; Characterization; Antioxidant activity; Immunostimulating activity
资金
- National Natural Science Foundation of China [31201454]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- [KYZ201218]
In this work, crude polysaccharides from Gracilaria rubra (GRPS) and its three purified fractions, GRPS-1-1, GRPS-2-1 and GRPS-3-2, were obtained through hot water extraction and chromatographic purification with DEAE-52 cellulose column and Sephadex G-50 column. The three purified fractions, being homogeneous heteropolysaccharides with average molecular weights of 1310, 691, 923 kD and sulfuric radical contents of 5.96, 8.46 and 12.03%, respectively, were all composed of galactose and fucose. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. Crude GRPS and its purified fractions exhibited strong scavenging activities against ABTS and superoxide radicals and lipid peroxidation inhibition. At a cellular level, GRPS-3-2 showed the strongest protective effect on H2O2-induced oxidative injury in PC12 cells. Moreover, GRPS-3-2 exhibited a much stronger immunostimulating activity on RAW264.7 cells than GRPS-1-1 and GRPS-2-1. The results suggested that GRPS, especially GRPS-3-2, could be developed as a promising functional food supplement. (C) 2016 Elsevier Ltd. All rights reserved.
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