期刊
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷 45, 期 9, 页码 1360-1366出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S1872-2040(17)61039-9
关键词
Insulin; Molecular imprinting; Electrochemical sensor; Epitope imprinting
资金
- National Natural Science Foundation of China [21375031, 21765006]
- Natural Science Foundation of Guangxi Province, China [2015GXNSFFA139005]
- High Level Innovation Teams of Guangxi Colleges & Universities and Outstanding Scholars Program, China [Guijiaoren[2014]49]
A new molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. The molecularly imprinted polymer was fabricated by electropolymerization with C-terminal polypeptide of insulin self-assembled on the Au electrode as template molecule and o-PD as functional monomer. After elution of template molecules by NaOH solution, the imprinting cavities were formed with the three-dimensional structure matching with the C-terminal polypeptide of insulin molecule. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] as a probe, the insulin could be indirectly detected. There was a linear relationship between the response current and the concentration of insulin in the range of 1.0 x 10(-14). 5.0 x 10(-13) M, and the detection limit was 7.24 x 10(-15) M (3 sigma/S). The developed sensor exhibited good selectivity and stability; and was successfully applied to determination of insulin in serum samples with satisfactory results.
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