期刊
ELECTROPHORESIS
卷 38, 期 16, 页码 2081-2088出版社
WILEY
DOI: 10.1002/elps.201700057
关键词
Bacteria; Flow cytometry; HS-SPME-GC; MS; Saliva; Voc
资金
- National Science Centre, Poland [2014/ 14/A/ST4/00641, 2014/ 15/N/ST4/03702, 2016/21/B/ST4/02130]
The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24, and 48h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS). Oral bacteria were the most viable within 2h after collection of saliva. Extension of incubation time to 48h caused considerable decrease in live bacteria counts and sharp increase in dead bacteria counts. The most prevalent strain was Sphingomonas paucimobilis (26.67%). The number of volatiles raised from 5 to 27 with incubation time and most of them were putrefaction products, such as methanethiol, indole and pyrrole. HS-SPME-GC/MS method is insufficient for volatile profiling of fresh saliva and should be directed rather to investigation of bacterial metabolites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据