期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1859, 期 9, 页码 1526-1535出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2017.04.005
关键词
G-proteins; Membranes; Palmitoylation; Myristoylation; Cell signaling; Isoprenoids
资金
- Spanish Ministerio de Economia y Competitividad [BIO2010-21132, BIO2013-49006-C2-1-R, RTC-2015-3542-1, RTC-2015-4094-1]
- FEDER from the EU (Una manera de hacer Europa)
- Govern de les Illes Balears (Grups competitius and Research Excellent Grant)
- Marathon Foundation
- Ministerio de Ciencia e Innovation
- Ministerio de Education, Cultura y Deporte
- Spanish Ministerio de Economia y Competitividad
- Asociacion Espafiola Contra el Cancer
G proteins often bear myristoyl, palmitoyl and isoprenyl moieties, which favor their association with the membrane and their accumulation in G Protein Coupled Receptor-rich microdomains. These lipids influence the biophysical properties of membrdnes and thereby modulate G protein binding to bilayers. In this context, we showed here that geranylgeraniol, but neither myristate nor palmitate, increased the inverted hexagonal (Hu) phase propensity of phosphatidylethanolamine-containing membranes. While myristate and palmitate preferentially associated with phosphatidylcholine membranes, geranylgeraniol favored nonlamellar-prone membranes. In addition, G alpha i(1) monomers had a higher affinity for lamellar phases, while G beta gamma and G beta gamma showed a marked preference for nonlamellar prone membranes. Moreover, geranylgeraniol enhanced the binding of G protein dimers and trimers to phosphatidylethanolamine-containing membranes, yet it decreased that of monomers. By contrast, both myristate and palmitate increased the Gail preference for lamellar membranes. Palmitoylation reinforced the binding of the monomer to PC membranes and myristoylation decreased its binding to PE-enriched bilayer. Finally, binding of dimers and trimers to lamellar-prone membranes was decreased by palmitate and myristate, but it was increased in nonlamellar-prone bilayers. These results demonstrate that co/post-translational G protein lipid modifications regulate the membrane lipid structure and that they influence the physico-chemical properties of membranes, which in part explains why G protein subunits sort to different plasma membrane domains. This article is part of a Special Issue entitled: Membrane Lipid Therapy: Drugs Targeting Biomembranes edited by Pablo V. Escriba. (C) 2017 Elsevier B.V. All rights reserved.
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