期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2017.00361
关键词
calcitonin gene-related peptide (CGRP); alpha 9 nicotinic acetylcholine receptor; Ld'T alpha 9 nicotinic acetylcholine receptor; lateral olivocochlear efferent (LOC); medial olivocochlear efferent (MOC); hearing in noise; prepulse inhibition; acousticstartle
资金
- USPHS NIH-NIDCD [P30 DC005409, R01 DC003086]
- ARRA
Approximately 15% of American adults report some degree of difficulty hearing in a noisy environment or have auditory filtering difficulties. There are objective clinical tests of auditory filtering, yet few tests exist for mouse models that do not rely on extensive training. We have used reflex modification audiometry (RMA) and developed exclusion criteria for the mouse model. This RMA based test makes use of the acoustic startle response (ASR) and the ability of prepulses to inhibit the ASR [i.e., prepulse inhibition (PPI)] to assess the mouse's ability to detect prepulse signals presented in quiet or embedded in masking noise. We have studied PPI behavior across four inbred mouse strains with normal cochlear function and developed pre-testing exclusion criteria and test/retest reliability measures. Moreover, because both the medial (MOC) and the lateral (LOC) olivocochlear efferent feedback systems have been proposed to improve auditory behavior performance, especially in noisy backgrounds, we have examined PPI abilities in mice (with their littermate controls) either lacking the MOC receptor subunit a9 nicotinic acetylcholine receptor [a9 nAChR (-/-)] or expressing an overactive receptor [Ld'T mutation in a9 nAChR KI], or lacking an LOC efferent neuropeptide, alpha calcitonin gene-related peptide [aCGRP (-/-)] only in the CNS. Because CGRP receptor formation has been shown to mature from juvenile to adult ages, we also studied if this maturation would be reflected in PPI behavioral responses in juvenile and adult (+/+) controls and in adult aCGRP (-/-) animals. We show that 50% PPI response thresholds (sound level with 50% correct responses) in quiet are decreased in the (-/-) a9 nAChR animals, and 50% PPI responses are increased for mice with an overactive receptor (a9 nAChR KI) and are increased in adult mice lacking aCGRP (-/-). However, in background noise, only mice lacking aCGRP exhibited increased 50% PPI response thresholds, as there were no significant differences between a9 nAChR adult mouse lines and their littermate controls. These findings suggest that MOC and LOC olivocochlear neurotransmission work in tandem to improve behavioral responses to sound. These experiments further pave the way for rapid behavioral hearing assessments in other mouse models.
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