期刊
CANADIAN JOURNAL OF CHEMISTRY
卷 95, 期 8, 页码 837-844出版社
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/cjc-2017-0008
关键词
coacervation; amphiphilic copolymer; polysaccharide; complex formation
资金
- National Natural Science Foundation of China [31470434, 21406090, 21576124, 21676124]
- China Postdoctoral Science Foundation [2017M610308]
- Natural Research Project of the Jiangsu Higher Education Institutions of China [16KJB550010]
- Zhenjiang Social development project [SH2016019]
- Science Foundation of Jiangsu Entry-exit Inspection Quarantine Bureau [2016KJ51, 2017KJ47]
Heat-induced coacervation of triblock copolymer solution was described, and its application in the purification of Lycium barbarum polysaccharide (LBP) was investigated. The formation of coacervate micelles-protein complex combined with the incompatibility between coacervate micelles and polysaccharide made it an ideal system for the separation of protein and LBP. This separation process was governed by a series of parameters including polymer concentration, amount of crude LBP solution, and pH. In the primary coacervation extraction process, LBP was preferentially distributed to dilute phase with a high recovery ratio of 82%, whereas 87% of protein was partitioned to the coacervate phase. The coacervate micelles-protein interaction and the interphase potential was regulated by temperature and electrolytes, respectively, which contributed to the recovery and recycling of the polymer. After phase separation, LBP was precipitated with the addition of ethanol. The FTIR spectrum was used to identify LBP. In addition, the antioxidant activity of LBP was measured.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据