期刊
CARBOHYDRATE POLYMERS
卷 173, 期 -, 页码 732-748出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.06.031
关键词
Monostroma angicava Kjellm; Sulfated polysaccharide; Structure; Antidiabetic activity; Anticoagulant property
资金
- National Natural Science Foundation of China [41476108]
- Science and Technology Development Program of Shandong Province, China [2014GHY115015]
- NSFC-Shandong Joint Fund for Marine Science Research Centers [U1606403]
- Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology [2015ASKJ02]
A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of -> 3)-alpha-L-Rhap-(1 -> and -> 2)-alpha-L-Rhap-(1 -> residues, partially sulfated at C-2 of -> 3)-alpha-LRhap-(1 -> and C-3/C-4 of -> 2)-alpha-L-Rhap The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked alpha-L-rhamnose and terminal beta-D-glucuronic acid residues. The polysaccharide had strong antidiabetic activity assessed by glucose consumption, total cholesterol and triglyceride levels using human hepatocellular carcinoma (HepG2) and insulin-resistant HepG2 cells. The polysaccharide exhibited high anticoagulant property by activated partial thromboplastin time and thrombin time assays, and possessed high fibrin(ogen)olytic activity evaluated by plasminogen activator inhibitior-1, fibrin(ogen) degradation products and D-dimer levels using rats plasma. The investigation demonstrated that the polysaccharide from Monostroma angicava Kjellm was a novel sulfated rhamnan and could be a potential antidiabetic and anticoagulant polysaccharide. (C) 2017 Elsevier Ltd. All rights reserved.
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