期刊
ANALYTICAL CHEMISTRY
卷 89, 期 17, 页码 9048-9055出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01729
关键词
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资金
- National Institutes of Health [NIH R01GM092218]
- U.S. Army Research Office and Defense Advanced Research Projects Agency (DARPA) [W911 NF-14-2-0022]
- U.S. Environmental Protection Agency (EPA) [83573601]
- National Institute of Environmental Health Sciences of the NIH [R01 ES022190]
- DOE [DE-AC05-76RL0 1830]
- Vienna Business Agency
Collision cross section (CCS) measurements resulting from ion mobility mass spectrometry (IM-MS) experiments provide a promising orthogonal dimension of structural information in MS-based analytical separations. As with any molecular identifier, interlaboratory standardization must precede broad range integration into analytical workflows. In this study, we present a reference drift tube ion mobility mass spectrometer (DTIM-MS) where improvements on the measurement accuracy of experimental parameters influencing IM separations provide standardized drift tube, nitrogen CCS values ((CCSN2)-C-DT) for over 120 unique ion species with the lowest measurement uncertainty to date. The reproducibility of these (CCSN2)-C-DT values are evaluated across three additional laboratories on a commercially available DTIM-MS instrument. The traditional stepped field CCS method performs with a relative standard deviation (RSD) of 0.29% for all ion species across the three additional laboratories. The calibrated single field CCS method, which is compatible with a wide range of chromatographic inlet systems, performs with an average, absolute bias of 0.54% to the standardized stepped field (CCSN2)-C-DT values on the reference system. The low RSD and biases observed in this interlaboratory study illustrate the potential of DTIM-MS for providing a molecular identifier for a broad range of discovery based analyses.
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