期刊
BIO-PROTOCOL
卷 7, 期 17, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2533
关键词
Arabidopsis thaliana; Extracellular vesicles; EVs; Apoplastic wash; DiOC(6); Fluorometric quantification
类别
资金
- United States National Science Foundation [IOS-1645745]
Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves. The isolated EVs can be quantified using a fluorometric dye to assess total membrane content.
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