期刊
ANALYTICAL CHEMISTRY
卷 89, 期 17, 页码 8908-8916出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01379
关键词
-
资金
- Natural Science Foundation of China [21575098, 21505098, 11504259]
- Shanxi Provincial government (100-talents program)
- Shanxi province international cooperation project [2015081019]
- Shanxi Special Programs for Platforms and Talents [201605D211033]
- Natural Sciences and Engineering Research Council (NSERC) of Canada
We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional enzyme-linked immunosorbent assay (ELISA) kits were obtained. The achieved detection limit (3 +/- 1 ppb, equivalent to mu g/kg) and dynamic response range (0.5-250 ppb) meet the requested testing standards set by authorities in China and North America. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据