期刊
CHEMICAL SCIENCE
卷 8, 期 10, 页码 7236-7245出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7sc03448b
关键词
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资金
- National Institutes of Health [CA186798]
- Fred Hutchinson Cancer Research Center's STTR program
- University of Washington
One constraint of semiconducting polymer dots (Pdots), especially those with near-IR emission, is their low effective emitter ratio (similar to 1.5 mole percent), which limits their pH sensing performance. The other critical issue of existing Pdot-based pH sensors is their poor photostability. To address these issues, we developed a series of Pdots by dendronizing the squaraine-based pH responsive near-IR emitter, which is covalently incorporated into the polyfluorene (PFO) backbone. The fluorescence self-quenching of the NIR squaraine emitter was effectively suppressed at a high emitter concentration of 5 mole percent. Through controlling the individually incomplete energy transfer from the amorphous PFO donor to the blue beta-phase PFO and NIR squaraine emitter, we obtained a ratiometric pH sensor with simultaneously improved pH sensitivity, brightness, and photostability. The Pdots showed a fast and reversible pH response over the whole biological pH range of 4.7 to 8.5. Intracellular pH mapping was successfully demonstrated using this ultra-bright and photostable Pdot-based pH indicator.
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