期刊
CHEMICAL SCIENCE
卷 8, 期 3, 页码 2199-2203出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6sc04071c
关键词
-
资金
- National Key Basic Research Program of China [2014CB744503]
- National Natural Science Foundation of China [81501534, 21271030, 81471707]
- Fundamental Research Funds for the Central Universities [20720150063]
- Science Foundation of Fujian Province [2014Y2004]
A novel Forster resonance energy transfer (FRET) fluorescence off-on system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and[4'-(N,N'-dimethylamino) phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The in vivo imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of in vivo semiquantitative fluorescence imaging of NO in mammals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据