期刊
CHEMICAL SCIENCE
卷 8, 期 9, 页码 5909-5917出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7sc00758b
关键词
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资金
- ANR agency [Ln23 - ANR-13-BS07-0007-01]
- Grenoble Instruct Center (ISBG) [UMS 3518 CNRS-CEA-UGA-EMBL]
- French Infrastructure for Integrated Structural Biology Initiative FRISBI [ANR-10-INSB-05-02]
- Grenoble Alliance for Integrated Structural Cell Biology GRAL within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-LABX-49-01]
- [L-1382]
Macromolecular crystallography suffers from two major issues: getting well-diffracting crystals and solving the phase problem inherent to large macromolecules. Here, we describe the first example of a lanthanide complex family named crystallophore (Xo4), which contributes to tackling both bottlenecks. This terbium complex, Tb-Xo4, is an appealing agent for biocrystallography, combining the exceptional phasing power of the Tb(III) heavy atom with powerful nucleating properties, providing ready-to-use crystals for structure determination. Furthermore, protein/Tb-Xo4 co-crystals can be easily detected and discriminated from other crystalline by-products using luminescence. We demonstrate the potential of this additive for the crystallisation and structure determination of eight proteins, two of whose structures were unknown.
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