期刊
DEVELOPMENTAL CELL
卷 42, 期 5, 页码 542-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2017.07.014
关键词
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资金
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- Ellison Medical Foundation [AG-SS-2951-12]
- NIH [R01 GM114140]
- Kimmel Scholar Award
Kinase translocation reporters (KTRs) are genetically encoded fluorescent activity sensors that convert kinase activity into a nucleocytoplasmic shuttling equilibrium for visualizing single-cell signaling dynamics. Here, we adapt the first-generation KTR for extracellular signal-regulated kinase (ERK) to allow easy implementation in vivo. This sensor, ERK-nKTR, allows quantitative and qualitative assessment of ERK activity by analysis of individual nuclei and faithfully reports ERK activity during development and neural function in diverse cell contexts in Caenorhabditis elegans. Analysis of ERK activity over time in the vulval precursor cells, a well-characterized paradigm of epidermal growth factor receptor (EGFR)-Ras-ERK signaling, has identified dynamic features not evident from analysis of developmental endpoints alone, including pulsatile frequency-modulated signaling associated with proximity to the EGF source. The toolkit described here will facilitate studies of ERK signaling in other C. elegans contexts, and the design features will enable implementation of this technology in other multicellular organisms.
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