期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 23, 期 49, 页码 11891-11897出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201702208
关键词
analytical methods; biosensors; dyes; pigments; fluorescent probes; imaging agents
资金
- DST
- CSIR
- DBT-IISc
- Bio-imaging Facility, Division of Biological Sciences, IISc
An easily synthesized fluorescein-based luminescent dye has been utilized for the dual-mode detection of histamine at nanomolar concentrations at pH7.0 in water. The specific response to histamine was achieved by imidazole-catalyzed imine formation' reaction. The protocol was subsequently applied for the estimation of histamine in complex biological milieu such as human blood serum and urine samples. Furthermore, the dose-dependent cellular uptake of histamine and de novo synthesis (by thapsigargin treatment) was visualized in RAW 264.7, a mouse macrophage cell line. We have also developed portable paper strips for rapid, on-site detection of histamine without involving costly instruments.
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