期刊
CHEMBIOCHEM
卷 18, 期 17, 页码 1730-1734出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201700240
关键词
antigens; chemobacterial synthesis; peptides; sialyl-Tn; vaccine conjugates
资金
- LabEx ARCANE [ANR-11-LABX-0003-01]
A conjugatable form of the tumour-associated carbohydrate antigen sialyl-Tn (Neu5Ac--2,6-GalNAc) was efficiently produced in Escherichia coli. Metabolically engineered E.coli strains overexpressing the 6-sialyltransferase gene of Photobacterium sp. and CMP-Neu5Ac synthetase genes of Neisseria meningitidis were cultivated at high density in the presence of GalNAc--propargyl as the exogenous acceptor. The target disaccharides, which were produced on the scale of several hundreds of milligrams, were then conjugated by using copper(I)-catalysed azide-alkyne cycloaddition click chemistry to a fully synthetic and immunogenic scaffold with the aim to create a candidate anticancer vaccine. Four sialyl-Tn epitopes were introduced on the upper face of an azido-functionalised multivalent cyclopeptide scaffold, the lower face of which was previously modified by an immunogenic polypeptide, PADRE. The ability of the resulting glycoconjugate to interact with oncofoetal sialyl-Tn monoclonal antibodies was confirmed in ELISA assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据