期刊
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
卷 196, 期 6, 页码 772-781出版社
AMER THORACIC SOC
DOI: 10.1164/rccm.201706-1208OC
关键词
T-cell epitopes; Mycobacterium tuberculosis; tuberculosis; adaptive immunity; microbiome
资金
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [HHSN272200900044C, U19 AI118626, U19 AI111211]
- Bill and Melinda Gates Foundation [OPP1066265]
- Bill and Melinda Gates Foundation [OPP1066265] Funding Source: Bill and Melinda Gates Foundation
Rationale: Individuals with a history of tuberculosis (TB) disease are at elevated risk of disease recurrence. The underlying cause is not known, but one explanation is that previous disease results in less-effective immunity against Mycobacterium tuberculosis (Mtb). Objectives: We hypothesized that the repertoire of Mtb-derived epitopes recognized by T cells from individuals with latent Mtb infection differs as a function of previous diagnosis of active TB disease. Methods: T-cell responses to peptide pools in samples collected from an adult screening and an adolescent validation cohort were measured by IFN-gamma enzyme-linked immunospot assay or intracellular cytokine staining. Measurements and Main Results: We identified a set of type 2 T-cell epitopes that were recognized at 10-fold-lower levels in Mtb-infected individuals with a history of TB disease less than 6 years ago than in those without previous TB. By contrast, type 1 epitopes were recognized equally well in individuals with or without previous TB. The differential epitope recognition was not due to differences in HLA class II binding, memory phenotypes, or gene expression in the responding T cells. Instead, TB disease historysensitive type 2 epitopes were significantly (P, 0.0001) more homologous to sequences from bacteria found in the human microbiome than type 1 epitopes. Conclusions: Preferential loss of T-cell reactivity to Mtb epitopes that are homologous to bacteria in the microbiome in persons with previous TB disease may reflect long-term effects of antibiotic TB treatment on the microbiome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据