期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 39, 页码 11788-11792出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201705747
关键词
bio-imaging; fluorescent probes; in situ imaging; osteosarcoma; solid-state fluorescence
资金
- National Natural Science Foundation of China [21325520, 21521063, 21327009, J1210040]
- science and technology project of Hunan Province [2016RS2009, 2016WK2002]
Current enzyme-responsive, fluorogenic probes fail to provide insitu information because the released fluorophores tend to diffuse away from the reaction sites. The problem of diffusive signal dilution can be addressed by designing a probe that upon enzyme conversion releases a fluorophore that precipitates. An excited-state intramolecular proton transfer (ESIPT)-based solid-state fluorophore HTPQ was developed that is strictly insoluble in water and emits intense fluorescence in the solid state, with (ex/em)=410/550nm, thus making it far better suited to use with a commercial confocal microscope. HTPQ was further utilized in the design of an enzyme-responsive, fluorogenic probe (HTPQA), targeting alkaline phosphatase (ALP) as a model enzyme. HTPQA makes possible diffusion-resistant insitu detection of endogenous ALP in live cells. It was also employed in the visualizing of different levels of ALP in osteosarcoma cells and tissue, thus demonstrating its interest for the diagnosis of this type of cancer.
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