期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 23, 期 51, 页码 12646-12654出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201702649
关键词
cell recognition; fluorescent probes; liposomes; membranes; supramolecular chemistry
资金
- NSF [CHE1401783]
- NIH [R01GM059078, T32GM075762]
- CONACyT (Mexico)
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1401783] Funding Source: National Science Foundation
A new self-assembly method is used to rapidly functionalize the surface of liposomes without perturbing the membrane integrity or causing leakage of the aqueous contents. The key molecule is a cholesterol-squaraine-PEG conjugate with three important structural elements: a cholesterol membrane anchor, a fluorescent squaraine docking station that allows rapid and high-affinity macrocycle threading, and a long PEG-2000 chain to provide steric shielding of the decorated liposome. The two-step method involves spontaneous insertion of the conjugate into the outer leaflet of pre-formed liposomes followed by squaraine threading with a tetralactam macrocycle that has appended targeting ligands. A macrocycle with six carboxylates permitted immobilization of intact fluorescent liposomes on the surface of cationic polymer beads, whereas a macrocycle with six zinc(II)-dipicolylamine units enabled selective targeting of anionic membranes, including agglutination of bacteria in the presence of human cells.
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