期刊
BIOFOULING
卷 33, 期 8, 页码 624-639出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2017.1350655
关键词
Catheter associated; urinary tract infections; Pseudomonas aeruginosa; quorum sensing; virulence; factors eugenol; biofilm
资金
- Indian Council of Medical Research (ICMR), New Delhi [80/903/2014-ECD-1]
The primary objective of this study was to ascertain the anti-biofilm and anti-virulence properties of sub-minimum inhibitory concentration (MIC) levels of eugenol against the standard strain PAO1 and two multi-drug resistant P. aeruginosa clinical isolates utilizing quorum sensing inhibition (QSI). Eugenol at 400 mu M significantly reduced biofilm formation on urinary catheters and the virulence factors (VF) including extracellular polysaccharides, rhamnolipid, elastase, protease, pyocyanin, and pyoverdine (p < 0.001). Further, eugenol exhibited a marked effect on the production of QS signals (AIs) (p < 0.001) without affecting their chemical integrity. In silico docking studies demonstrated a stable molecular binding between eugenol and QS receptor(s) in comparison with respective AIs. Investigation on reporter strains confirmed the competitive binding of eugenol to a QS receptor (LasR) as the possible QSI mechanism leading to significant repression of QS associated genes besides the VF genes (p < 0.001). This study provides insights, for the first time, into the mechanism of the anti-virulence properties of eugenol.
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