期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 40, 页码 12132-12136出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201705684
关键词
gels; hyaluronic acid; matrix mechanics; photochemistry; stem cells
资金
- NIH [DE016523]
- Center for Engineering MechanoBiology (NSF STC program) [CMMI: 15-48571]
- BWF [1015895]
- HHMI
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1548571] Funding Source: National Science Foundation
The relationship between ECM mechanics and cell behavior is dynamic, as cells remodel and respond to changes in their local environment. Most invitro substrates are static and supraphysiologically stiff; thus, platforms with dynamic and reversible mechanical changes are needed. Herein, we developed hyaluronic acid-based substrates capable of sequential photodegradation and photoinitiated crosslinking reactions to soften and then stiffen the hydrogels over a physiologically relevant range of moduli. Reversible mechanical signaling to adhered cells was demonstrated with human mesenchymal stem cells. Insitu hydrogel softening (from ca. 14 to 3.5kPa) led to a decrease in the cell area and nuclear localization of YAP/TAZ, and subsequent stiffening (from ca. 3.5 to 28kPa) increased the cell area and nuclear localization of YAP/TAZ. Each photoreaction was cytocompatible and tunable, rendering this platform amenable to studies of dynamic mechanics on cell behavior across many cell types and contexts.
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