期刊
BIOCHIMIE
卷 140, 期 -, 页码 117-121出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2017.07.005
关键词
Cystatin; Cysteine proteases; Tick; Midgut
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/03657-8, 2011/09340-3]
- Conselho Nacional de Desenvolvimento Tecnologico (CNPq) [308780/2013-2, 313073/2013-9]
- CAPES/Brazil (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- INCT-Entomologia Molecular (CNPq) [465678/2014-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/03657-8] Funding Source: FAPESP
The Rhipicephalus (Boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. Cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. Recently, our group performed a midgut transcriptome of engorged R. microplus tick, and out of 800-ESTs sequences one cystatin-coding sequence was identified and named Rmcystatin-4. In order to understand the physiological role of Rmcystatin-4, the aim of this work was the expression, purification and functional characterization of a novel type 2 cystatin from the tick R. microplus. Rmcystatin-4 gene expression was identified mostly in tick midgut suggesting its possible role in blood digestion control. Our data showed that rRmcystatin-4 was successfully expressed in active form using Pichia pastoris system and the purified inhibitor presented high selectivity to BmCl-1 (Ki = 0.046 nM). Moreover, rRmcystatin-4 was able to impaired BmCl-1 activity towards bovine hemoglobin. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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