期刊
BIOCHEMISTRY
卷 56, 期 36, 页码 4751-4756出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.7b00711
关键词
-
资金
- National Institutes of Health [R01 GM087934]
The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 angstrom using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of micro crystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.
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