期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 27, 期 18, 页码 4285-4287出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2017.08.041
关键词
Binding-elution; Cation exchange; Human milk oligosaccharides; Enzymatic synthesis; Amino linker
资金
- Key Grant Project of Chinese Ministry of Education [313033]
- National Natural Science Foundation of China [31200605]
A cation exchange assisted binding-elution (BE) strategy for enzymatic synthesis of human milk oligosaccharides (HMOs) was developed. An amino linker was used to provide the cation ion under acidic condition which can be readily bound to cation exchange resin and then eluted off by saturated ammonium bicarbonate. Ammonium bicarbonate in the collections was easily removed by vacuum evaporation. This strategy circumvented the incompatible issue between glycosyltransferases and solid support or large polymers, and no purification was needed for intermediate products. With current approach, polyLacNAc backbones of HMOs and fucosylated HMOs were synthesized smoothly. (C) 2017 Elsevier Ltd. All rights reserved.
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