期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 27, 期 18, 页码 4431-4435出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2017.08.010
关键词
Nocamycins; Methyltransferase; Post-tailoring modification; Enzyme reaction; Antibacterial activity
资金
- National Natural Science Foundation of China [31300063]
- Natural Science Foundation of Shandong Province [ZR2013CL020]
- Research Foundation for Advanced Talents of Qingdao Agricultural University [631301]
- Open Research Foundation of CAS Key Laboratory of Tropical Marine Bio-resources and Ecology [LMB141007]
Nocamycins belong to the tetramic acid family natural products and show potent antimicrobial activity. Recently, the biosynthetic gene cluster of nocamycin was identified from the rare actinomycete Saccharothrix syringae and an S-adenosylmethionine (SAM) dependent methyltransferase gene NcmP was found to be located within the gene cluster. In this report, the methyltransferase gene NcmP was disrupted and a new nocamycin intermediate nocamycin E was isolated from the mutant strain. Meanwhile, NcmP was heterologously expressed in Escherichia coli BL21 (DE3) and biochemically characterized as a carboxylate O-methyltransferase in nocamycin biosynthetic pathway. Compared to nocamycin I, nocamycin E showed inferior antibacterial activity, indicating the methyl group is essential to antibacterial activity. (C) 2017 Published by Elsevier Ltd.
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