4.5 Article

Effects of prostaglandin lipid mediators on agonist-induced lung endothelial permeability and inflammation

出版社

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00519.2016

关键词

pulmonary endothelial cells; permeability; inflammation; prostaglandins; acute lung injury

资金

  1. National Heart, Lung, and Blood Institute Grants [HL-076259, HL-087823, HL-107920]
  2. National Institute of General Medical Sciences Grant [GM-114171]

向作者/读者索取更多资源

Prostaglandins (PG), the products of cyclooxygenase-mediated conversion of arachidonic acid, become upregulated in many situations including allergic response, inflammation, and injury, and exhibit a variety of biological activities. Previous studies described barrier-enhancing and anti-inflammatory effects of PGE(2) and PGI(2) on vascular endothelial cells (EC). Yet, the effects of other PG members on EC barrier and inflammatory activation have not been systematically analyzed. This study compared effects of PGE(2), PGI(2), PGF(2 alpha), PGA(2), PGJ(2), and PGD(2) on human pulmonary EC. EC permeability was assessed by measurements of transendothelial electrical resistance and cell monolayer permeability for FITC-labeled tracer. Antiinflammatory effects of PGs were evaluated by analysis of expression of adhesion molecule ICAM1 and secretion of soluble ICAM1 and cytokines by EC. PGE(2), PGI(2), and PGA(2) exhibited the most potent barrier- enhancing effects and most efficient attenuation of thrombininduced EC permeability and contractile response, whereas PGI(2) effectively suppressed thrombin-induced permeability but was less efficient in the attenuation of prolonged EC hyperpermeability caused by interleukin-6 or bacterial wall lipopolysaccharide, LPS. PGD(2) showed a modest protective effect on the EC inflammatory response, whereas PGF(2 alpha) and PGJ(2) were without effect on agonist-induced EC barrier dysfunction. In vivo, PGE(2), PGI(2), and PGA(2) attenuated LPS-induced lung inflammation, whereas PGF(2 alpha) and PGJ(2) were without effect. Interestingly, PGD(2) exhibited a protective effect in the in vivo model of LPS-induced lung injury. This study provides a comprehensive analysis of barrier-protective and anti-inflammatory effects of different prostaglandins on lung EC in vitro and in vivo and identifies PGE(2), PGI(2), and PGA(2) as prostaglandins with the most potent protective properties.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据