期刊
CARDIOVASCULAR THERAPEUTICS
卷 35, 期 5, 页码 -出版社
WILEY-HINDAWI
DOI: 10.1111/1755-5922.12277
关键词
Chronic heart failure; Doxorubicin; Oxidative stress; Sulforaphane
资金
- National Science Foundation of China [81300186, 81301035]
- Science and Technology Development Project Youth Foundation of Jilin Province [20150520147JH]
BackgroundDoxorubicin (DOX) is an anthracycline antitumor drug. However, its clinical use is limited by dose-dependent cardiotoxicity and even progresses to chronic heart failure (CHF). ObjectiveThis study aims to investigate whether the Nrf2 activator, sulforaphane (SFN), can prevent DOX-induced CHF. MethodsMale Sprague-Dawley rats which received treatment for 6weeks were divided into four groups (n=30 per group): control, SFN, DOX and DOX plus SFN group. ResultsResults revealed that DOX induced progressive cardiac damage as indicated by increased cardiac injury markers, cardiac inflammation, fibrosis and oxidative stress. SFN significantly prevented DOX-induced progressive cardiac dysfunction between 2-6weeks and prevented DOX-induced cardiac function deterioration. Furthermore, it significantly decreased ejection fraction and increased the expression of brain natriuretic peptide. SFN also almost completely prevented DOX-induced cardiac oxidative stress, inflammation and fibrosis. SFN upregulated NF-E2-related factor 2 (Nrf2) expression and transcription activity, which was reflected by the increased mRNA expression of Nrf2 and its downstream genes. Furthermore, in cultured H9c2 cardiomyocytes, the protective effect of SFN against DOX-induced fibrotic and inflammatory responses was abolished by Nrf2 silencing. ConclusionWe arrived at the conclusion that DOX-induced CHF can be prevented by SFN through the upregulation of Nrf2 expression and transcriptional function.
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