期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 40, 期 10, 页码 1529-1542出版社
SPRINGER
DOI: 10.1007/s00449-017-1809-8
关键词
Silver nanoparticles; Low-shear-modeled microgravity; HARV; In vitro cytotoxicity; Bactericidal activity
资金
- National Research foundation of Korea (NRF) Grant - Korea Government [1201002622]
- Chonbuk National University
- university research grants from the Chonbuk National University
Gravitational force and shear forces induce various changes in gene expression and metabolite production of microorganisms. Previous reports have shown that there are differences in the expression of different sets of proteins and enzymes under microgravity conditions compared to normal gravity. The aim of this study is to utilize culture filtrates of Penicillium chrysogenum grown under microgravity and normal conditions to synthesize silver nanoparticles and to examine whether there is any difference between their physiochemical and biological function. Synthesized nanoparticles were characterized using UV-Vis spectroscopy, FTIR, XRD, and TEM. Biological functional studies such as antimicrobial activity, cytotoxic studies, and anticancer activity were carried out. Antimicrobial activity was tested using antibiotic susceptibility testing by Kirby-Bauer method and cytotoxicity tests were carried out using 3T3-L1 normal fibroblasts cells and Hep-G2 cancer cell lines. Interestingly, our results indicated that microgravity-synthesized silver nanoparticles possess enhanced antibacterial activity and cytotoxic effect against cancer cells compared to normal gravity-synthesized silver nanoparticle. This work highlighted the importance of gravitational vector on the fungal enzyme profiles and their role in silver nanoparticle synthesis with enhanced biological activity.
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