期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 33, 期 6, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-017-2278-6
关键词
Sporotrichum thermophile BJAMDU5; Hydrolytic enzymes; Multiple substrates; Oligosaccharides; Bread making
资金
- Council of Scientific and Industrial Research, New Delhi, India [38(1370)/13/EMR-II]
Sporotrichum thermophile BJAMDU5 secreted high titres of xylanolytic and cellulolytic enzymes in solid state fermentation using mixture of wheat straw and cotton oil cake (ratio 1: 1) at 45 C-omicron, pH 5.0 after 72 h inoculated with 2.9 x 10(7) CFU/mL conidiospores. Supplementation of solid medium with lactose and ammonium sulphate further enhanced the production of hydrolytic enzymes. Among different surfactants studied, Tween 80 enhanced the production of all enzymes [3455 U/g DMR (dry mouldy residue), 879.26 U/g DMR, 976.28 U/g DMR and 35.10 U/g DMR for xylanase, CMCase (Carboxymethylcellulase), FPase (Filter paper activity) and beta-glucosidase, respectively] as compared to other surfactants. Recycling of solid substrate reduced the production of all these enzymes after second cycle. End products analysis by TLC showed the ability of hydrolytic enzymes of S. thermophile to liberate monomeric (xylose and glucose) as well as oligomeric (xylobiose, cellobiose and higher ones) sugars. Supplementation of enzyme resulted in improved nutritional properties of the bread. Formation of oligomeric sugars by xylanase enzyme of S. thermophile BJAMDU5 make it a good candidate in food industry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据