Influence of sublethal doses of acetamiprid and halosulfuron-methyl on metabolites of zebra fish (&ITBrachydanio rerio&IT)

Acetamiprid, a neonicotinoid pesticide, is reported to have adverse sublethal effects on non-target beneficial organisms. Halosulfuron-methyl (HM), one of the most widely used herbicides in agriculture, has high ecotoxicity to aquatic plants and animals. In this study, a GC-MS-based metabolomics approach was used to in vestigate the toxicity of acetamiprid and HM. The Automated Mass Spectral Deconvolution and Identification System (AMDIS) software program and the retention index method were used to identify 51 metabolites in zebra fish (Brachydanio rerio). Changes in metabolites showed that acetamiprid and HM disturbed amino acid (e.g.,leucine, valine, serine, glycine, proline, and alanine) metabolism, the TCA cycle (malic acid and fumaric acid), and the balance of neurotransmitters (glutamic acid, taurine, and glycine). The change in metabolites in the liver, head, and blood indicated that metabolites in the liver were more sensitive than those in the head and blood. Overall, on the basis of the change in metabolites, we identified a potential risk to zebra fish exposed to sublethal doses of acetamiprid and/or HM.