期刊
BIOCHEMISTRY
卷 56, 期 39, 页码 5165-5170出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.7b00529
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资金
- Howard Hughes Medical Institute
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the photon budget require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, protein engineering, and organic synthesis that have allowed a triumphant return of chemical fluorophores to modern biological imaging.
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