期刊
BIOSENSORS & BIOELECTRONICS
卷 96, 期 -, 页码 113-120出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.04.050
关键词
Electrochemiluminescence biosensor; Quenching; Chitosan/Ru(bpy)(3)(2+)/silica/Fe3O4 nanomaterials; Glucose functionalized NiCo2S4 nanoparticles grown on carboxylic graphene; Specific carbohydrate-Con A interaction
类别
资金
- Natural Science Foundation of China [21375047, 21377046, 21575050, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- UJN [ts20130937]
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy)(3)(2+)/silica/Fe3O4 nanomaterials (CRuSi-Fe3O4) were synthesized through W/O microemulsion route. The added Fe3O4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe3O4. In addition, the layered structure of CRuSi-Fe3O4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo2S4 nanoparticles- grown on carboxylic graphene (NiCo2S4-COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo2S4 was used to quench the ECL of CRuSi-Fe3O4, graphene was used to grow NiCo2S4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S2O82- as the coreactant of CRuSi-Fe3O4. Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL(-1) to 100 ng mL(-1) was obtained, and the detection limit was calculated to be 0.18 pg mL(-1) (S/ N = 3).
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