期刊
DEVELOPMENTAL BIOLOGY
卷 430, 期 2, 页码 337-345出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2017.08.031
关键词
Master sex-determinant; Dmrt; DMY; Transactivation domain; Crustacea
资金
- Australian Research Council via a Discovery Early Career Research Award [DE130101089]
- USC Collaborative Research Networks (CRN) grant program
- University of the Sunshine Coast International Research Scholarship
- Industrial Transformation Research Hub [IH 120100032]
- [DP160103320]
- Australian Research Council [DE130101089] Funding Source: Australian Research Council
Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Y-linked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sex determining period during which iDMY > iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C'-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.
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