期刊
BIOSENSORS & BIOELECTRONICS
卷 96, 期 -, 页码 239-245出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.04.052
关键词
Photoelectrochemical immunosensor; Sandwich-type; WO3-Au; RGO-Ca:CdSe; Prostate specific antigen
类别
资金
- National Natural Science Foundation of China [21627809, 21575050, 21375047, 21377046, 21601064, 21405059]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004, ZR2016BQ10]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- UJN [ts20130937]
An ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor was constructed for the detection of prostate specific antigen (PSA). In this work, Au-nanoparticle-loaded tungsten oxide (WO3-Au) hybrid composites was applied as PEC sensing platform, while Ca ions doped CdSe equipped on the conducting framework of reduced graphene oxide (rGO-Ca:CdSe) nanocomposites were employed as the signal amplification probe. As for WO3-Au, massive Au nanoparticles were formed on the surface of WO3 without any additional reducing agent, providing a novel nanocarriers for anchoring plenty of the primary antibodies due to the large specific surface area and good biocompatibility by chemical bonding between Au nanoparticles and -NH2 of antibodies. Besides, the incorporation of the rGO and the doping of Ca ions could improve the conductivity and hinder the recombination of electron-hole pairs of CdSe nanoparticles effectively, thereby enhancing the photocurrent conversion efficiency. Based on the sandwich immunoreaction, the primary antibody was immobilized onto WO3-Au substrate, after the formed rGO-Ca:CdSe labels were captured onto the electrode surface via the specific antibody-antigen interaction, the photocurrent intensity could be further enhanced due to the sensitization effect. Under the optimal conditions, the proposed PEC immunosensor shows a linear relationship between photocurrent variation and the logarithm of PSA concentration in the wide range of 5 pg mL(-1) to 50 ng mL(-1) with a low detection limit of 2.6 pg mL(-1) (S/N=3). Moreover, it also presented good stability and acceptable specificity, indicating the potential applications in clinical diagnostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据