期刊
CELL
卷 171, 期 3, 页码 668-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2017.08.043
关键词
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资金
- European Research Council
- NIH [R01HD085121]
- Human Frontier Science Program [RGP0051]
- French Ministry of Higher Education and Research
- FRM [FDT20140930947]
- Schlumberger Foundation
- MacArthur Fellowship
The periodic segmentation of the vertebrate body axis into somites, and later vertebrae, relies on a genetic oscillator (the segmentation clock) driving the rhythmic activity of signaling pathways in the presomitic mesoderm (PSM). To understand whether oscillations are an intrinsic property of individual cells or represent a population-level phenomenon, we established culture conditions for stable oscillations at the cellular level. This system was used to demonstrate that oscillations are a collective property of PSM cells that can be actively triggered in vitro by a dynamical quorum sensing signal involving Yap and Notch signaling. Manipulation of Yap-dependent mechanical cues is sufficient to predictably switch isolated PSM cells from a quiescent to an oscillatory state in vitro, a behavior reminiscent of excitability in other systems. Together, our work argues that the segmentation clock behaves as an excitable system, introducing a broader paradigm to study such dynamics in vertebrate morphogenesis.
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