期刊
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
卷 45, 期 6, 页码 528-536出版社
WILEY
DOI: 10.1002/bmb.21066
关键词
Protein-protein interactions; molecular visualization; guided inquiry; laboratory exercises; noncovalent interactions
资金
- National Science Foundation [MCB 1552113, 1432466]
- Miami University
- Direct For Education and Human Resources
- Division Of Undergraduate Education [1432466] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1552113] Funding Source: National Science Foundation
Students' understandings of foundational concepts such as noncovalent interactions, pH and pK(a) are crucial for success in undergraduate biochemistry courses. We developed a guided-inquiry activity to aid students in making connections between noncovalent interactions and pH/pK(a). Students explore these concepts by examining the primary and tertiary structures of immunoglobulin G (IgG) and Protein A. Students use PyMOL, an open source molecular visualization application, to (1) identify hydrogen bonds and salt bridges between and within the proteins at physiological pH and (2) apply their knowledge of pH/pK(a) to association rate constant data for these proteins at pH 4 and pH 11. The laboratory activity was implemented within a one semester biochemistry laboratory for students majoring in allied health disciplines, engineering, and biological sciences. Several extensions for more advanced students are discussed. Students' overall performance highlighted their ability to successfully complete tasks such as labeling and identifying noncovalent interactions and revealed difficulties with analyzing noncovalent interactions under varying pH/pK(a) conditions. Students' evaluations after completing the activity indicated they felt challenged but also recognized the potential of the activity to help them gain meaningful understanding of the connections between noncovalent interactions, pH, pK(a), and protein structure. (C) 2017 by The International Union of Biochemistry and Molecular Biology.
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