期刊
BIORESOURCE TECHNOLOGY
卷 244, 期 -, 页码 929-933出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.07.185
关键词
Fumaric acid; Rhizopus arrhizus; Stirred-tank reactor; Fermentation
资金
- National Key research program [2016YFD0400601, 2017YFD0400603, 2017YFB0306900]
- Natural Science Foundation of China [21476017]
- Hong Kong, Macao and Taiwan scientific and technological cooperation projects [2015DFT30050]
- Amoy Industrial Biotechnology R & D and Pilot Conversion Platform [3502Z20121009]
- Fundamental Research Funds for the Central Universities [PYBZ1712]
Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20 g addition of loofah fiber and 400 rpm agitation speed were chosen as the most suitable process conditions. 30.3 g/L fumaric acid in the broth as well as 19.16 g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211 g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor.
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