期刊
ACS NANO
卷 11, 期 11, 页码 11264-11272出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b05631
关键词
artificial light harvesting; homo-FRET; DNA origami; photonic wires; energy transfer; single-molecule FRET
类别
资金
- European Commission through ERC, ORCA [336440]
- Volkswagen foundation
- Deutsche Forschungsgemeinschaft (DFG) [SFB1032]
- Ludwig-Maximillians-Universitat Munchen via the Center for NanoScience Munich (CeNS)
- LMUinnovativ initiative BioImaging Network (BIN)
Elaborating efficient strategies and deepening the understanding of light transport at the nanoscale is of great importance for future designs of artificial light-harvesting assemblies and dye-based photonic circuits. In this work, we focus on studying the phenomenon of Forster resonance energy transfer (FRET) among fluorophores of the same kind (homo-FRET) and its implications for energy cascades containing two or three different dye molecules. Utilizing the spatial programmability of DNA origami, we arranged a chain of cyanine 3 (Cy3) dyes flanked at one end with a dye of lower excitation energy, cyanine 5 (Cy5), with or without an additional dye of higher excitation energy, Alexa488, at the other end. We characterized the response of our fluorophore assemblies with bulk and single-molecule spectroscopy and support our measurements by Monte Carlo modeling of energy transfer within the system. We find that, depending on the arrangement of the fluorophores, homo-FRET between the Cy3 dyes can lead to an overall enhanced energy transfer to the acceptor fluorophore. Furthermore, we systematically analyzed the homo-FRET system by addressing the fluorescence lifetime and anisotropy. Finally, we built a homo-FRET-mediated photonic wire capable of transferring energy through the homo-FRET system from the blue donor dye (Alexa488) to the red acceptor fluorophore (Cy5) across a total distance of 16 nm.
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